Rapid and accurate identification of microorganisms remains an important challenge in microbiology. Despite all the numerous methods that are available nowadays, a good combination of simplicity, sensitivity and fast response is yet to be met. This problem can be solved by using highly performing chemiluminescent AquaSpark™ probes, as we have described in our latest scientific product minireview.
First publication date: September 2018
Rapid and sensitive pathogens detection is of paramount importance in the food and the pharmaceutical industries as well as in the public health system. Bioluminescence offers considerably higher sensitivity in comparison to chromogenic and fluorescence detection methods. In this new product minireview we highlight the general working principles of ProLuciferins (ProLucs), their uses for bioluminescent bacteria detection and other popular applications including the monitoring of cellular signaling pathways.
First publication date: April 2018
This minireview highlights the fascinating chemistry of dioxetanes and their exceptional light emission properties. Special attention is given to recent discoveries in the field which changed the game of chemiluminescence.
Latest update: April 2018
First publication date: January 2018
The review covers basic science of Luciferase enzymes, mechanistic aspects of FLuc bioluminescence, S.A.R of Luciferins and top-notch discoveries in the field. Particular attention is given to the highly promising area of in vivo bioluminescence.
Latest update: April 2018
First publication date: September 2017
In this product minireview we discuss the basic science of Luminol Chemiluminescence, its applications and the main areas of innovation in the field.
Latest update: April 2018
First publication date: June 2017
This product microreview focuses on the biosynthesis of nicotinamide riboside (NR) and its biological effects on the NAD+ metabolism. NR appears to be a promising candidate for the treatment of numerous diseases and as a food supplement.
First publication date: February 2018
Advanced techniques have been developed for the detection and differentiation of bacteria based on chromogenic and fluorogenic enzyme substrates. These sensitive methods have led to improved accuracy and faster detection of bacteria. This Application Note presents general procedures to detect specific bacteria enzymes using BIOSYNTH’s chromogenic or fluorogenic substrates in standard culture media like Nutrient Broth, Mueller-Hinton or Tryptic Soy media.
We are constantly developing our patented ALDOL® substrates; we explore additional applications for this platform and continue to optimize existing protocols. One of our recent findings shows that ALDOL® substrates inhibit the growth of certain Gram positive bacteria. To eliminate the inhibitory effect, we recommend adding a low concentration of Tween® 80 to the growth media for any applications aiming at the differentiation of Gram positive bacteria.
Classically, enterococci are detected using kanamycin-aesculin-azide media. Aesculin is hydrolysed by beta-glucosidase to glucose and aesculetin, the latter forms a brown-black coloured compound with ferric ions.
More recently, chromogenic and fluorogenic substrates for identification of Enterococcus spp. were developed, including a substrate for a second characteristic enzyme of these bacteria, pyroglutamyl aminopeptidase (L-Pyroglutamic acid 7-amido-4-methylcoumarin).
A powerful chromogenic substrate for the detection of Staphylococcus aureus
selectively by enzyme-triggered inhibition
Four innovative chromogenic enzyme substrates
Bright signals and high chemical stability
for the detection of E. coli and coliforms
Coelenterazine, Dioxetanes, Luciferin, Lucigenin, Luminol
Enzyme-Activated Inhibitor
Diagnostics, Isolation, Identification, Cultivation
Auxins, Cytokinins, Giberrellins (GA3), Abscisic Acid (ABA)
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